Hepatocyte Growth Factor, Recombinant, Human (HGF, Scatter Factor, SF, Hepatopoietin A)

HGF is a mesenchymally derived potent mitogen for mature parenchymal hepatocyte cells and acts as a growth factor for a broad spectrum of tissues and cell types. HGF signals through a transmembrane tyrosine kinase receptor known as MET. Activities of HGF include induction of cell proliferation, motility, morphogenesis, inhibition of cell growth, and enhancement of neuron survival. HGF is a crucial mitogen for liver regeneration processes, especially after partial hepatectomy and other liver injuries. Human and murine HGF are cross-reactive. Human HGF is expressed as a linear 697 amino acid polypeptide precursor glycoprotein. Proteolytic processing of this precursor generates the biologically active form of HGF, which consists of two polypeptide chains (alpha-chain and beta-chain) held by a single disulfide bond resulting in formation of a biologically active heterodimer. The alpha-chain consists of 463 amino acid residues and four kringle domains. The beta-chain consists of 234 amino acid residues.\n\nBiological Activity: \nThe ED50 was determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells was found to be in the range of 20-40ng/ml. \n\nAA Sequence:\nalph achain: QRKRRNTIHE FKKSAKTTLI KIDPALKIKT KKVNTADQCA NRCTRNKGLP FTCKAFVFDK ARKQCLWFPF NSMSSGVKKE FGHEFDLYEN KDYIRNCIIG KGRSYKGTVS ITKSGIKCQP WSSMIPHEHS FLPSSYRGKD LQENYCRNPR GEEGGPWCFT SNPEVRYEVC DIPQCSEVEC MTCNGESYRG LMDHTESGKI CQRWDHQTPH RHKFLPERYP DKGFDDNYCR NPDGQPRPWC YTLDPHTRWE YCAIKTCADN TMNDTDVPLE TTECIQGQGE GYRGTVNTIW NGIPCQRWDS QYPHEHDMTP ENFKCKDLRE NYCRNPDGSE SPWCFTTDPN IRVGYCSQIP NCDMSHGQDC YRGNGKNYMG NLSQTRSGLT CSMWDKNMED LHRHIFWEPD ASKLNENYCR NPDDDAHGPW CYTGNPLIPW DYCPISRCEG DTTPTIVNLD HPVISCAKTK QLR\n\nbeta chain: VVNGIPTRTN IGWMVSLRYR NKHICGGSLI KESWVLTARQ CFPSRDLKDY EAWLGIHDVH GRGDEKCKQV LNVSQLVYGP EGSDLVLMKL ARPAVLDDFV STIDLPNYGC TIPEKTSCSV YGWGYTGLIN YDGLLRVAHL YIMGNEKCSQ HHRGKVTLNE SEICAGAEKI GSGPCEGDYG GPLVCEQHKM RMVLGVIVPG RGCAIPNRPG IFVRVAYYAK WIHKIILTYK VPQS\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Reconstitute by adding sterile ddH2O and a carrier protein. Aliquot and store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C with a carrier protein. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nCountry of Origin: USA

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SPECIFICATIONS

Catalog Number

H2005-01

Size

2ug

Form

Supplied as a lyophilized powder from 0.1M L-arginine HCl, 10mM Tris, 200mM sodium chloride, pH 8.0. Reconstitute with sterile ddH2O and a carrier protein to 0.5mg/ml. Do not vortex

Purity

(same/more than) 98% by SDS-PAGE and HPLC analyses. Endotoxin: (same/less than) 0.1ng/ug (1EU/ug)

References

US Biological application reference: 1. Ziel., M. et al., (2007) Exp Hematol. 35:1550-1561. 2. Zhang, L. et al., (2011) Anal. Biochem. doi:10.1016/j.ab.2011.01.028. 1. Koch, A.: The SH2-domian-containing inositol 5-phosphatase (SHIP)-2 binds to c-Met directly via tyrosine residue 1356 and involves hepatocyte growth factor (HGF)-induced lamellipodium formation, cell scattering and cell spreading. Oncogene 24(21): 3436-47 (2005). 2.Suzuki, M.: Des-gamma-carboxy prothrombin is a potential autologous growth factor for hepatocellular carcinoma. Journal of Biological Chemistry 280(8): 6409-15 (2/25/2005). 3. Kawamura, K.: N-WASP and WAVE2 acting downstream of phosphatidylinositol 3-kinase are required for myogenic cell migration induced by hepatocyte growth factor. Journal of Biological Chemisstry 279(52): 54862-71 (12/24/2004).