Hepatocyte Specific Antigen

This antibody recognizes an uncharacterized antigen found in normal adult and fetal hepatocytes and hepato- cellular carcinomas. Tissue staining appears as a dis- tinct granular cytoplasmic pattern. This antibody may help in identifying hepatocytes in poorly defined tissues.\n\nApplications: \nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (Paraffin): 1:50 for 1 hr at RT\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nNormal adult human liver; hepatocytes (cytoplasmic staining pattern)\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H2007-01B

Size

1ml

Applications

IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder in 15mM sodium azide. Reconstitute with 1ml dH2O.

P Type

Mab

Purity

Supernatant

Isotype

IgG1,k

References

1.Fasano M, Theise N D, Nalesnik M, et al.. Immunohistochemical evaluation of hepatoblastomas with use of the hepatocyte-specific marker, hepatocyte paraffin 1, and the polyclonal anti-carcinoembryonic antigen. Modern Pathology. 11(10):934-938 (1998). 2.Schleger C, Heck R, Niketeghad F, et al.. Establishment and characterization of a nontumorigenic cell line derived from a human hepatocellular adenoma expressing hepatocyte-specific markers. Exp. Cell Res. 236(2):418-426 (1997). 3.Wennerberg A E, Nalesnik M A and Coleman W B. Hepatocyte paraffin 1: a monoclonal antibody that reacts with hepatocytes and can be used for differential diagnosis of hepatic tumors. American Journal of Pathology. 143:1050-1054 (1993).

Additional Info

Recognizes human Hepatocyte Specific Antigen.