Hepsin, Recombinant, Human (TMPRSS1)

Hepsin, also known as TMPRSS1, is a Type II membrane protein with an extracellular serine protease domain (1). It is most highly expressed in liver, but is also present in many other tissues, notably lung, kidney, and skeletal muscle (2). A soluble form of Hepsin lacking the transmembrane domain has been identified (3). Hepsin is capable of activating Factor VII, and may initiate blood coagulation at the cell surface (4). Hepsin is overexpressed in various human tumors, including prostate (5), and is considered to be a biomarker for prostate cancer (6). rhHepsin was expressed as a secreted, soluble protein lacking its cytosolic and transmembrane domains. The D161E and R162K mutations were introduced into the prosequence to improve expression of the rhHepsin. \n\nSource: \nRecombinant human Hepsin (aa residues 45-17 (D161E, R162K); P05981) was expressed with a C-terminal 10X His tag in a murine myeloma cell line, NS0. \n\nMolecular Mass: \nThe purified rhHepsin has N-terminal sequences of R45SDQEPLYPV, which corresponds to the proform. The 383aa residue rhHepsin predicts a molecular mass of 42kD. By \nSDS-PAGE under reducing conditions the protein migrates as multiple bands with molecular \nmasses of approximately 40-43kD. \n\nActivity: \nMeasured under the described conditions, is > 20,000 pmole/min/ug. \n\nStorage and Stability:\n12 months from date of receipt, 20 to 70 degrees C as supplied.\n1 month, 2 to 8 degrees C under sterile conditions after reconstitution.\n3 months, 20 to 70 degrees C under sterile conditions after reconstitution.\nUse a manual defrost freezer and avoid repeated freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

H2030-21C

Size

10ug

Form

Supplied as a 0.2um sterile-filtered solution in 50mM sodium acetate, 1M sodium chloride, pH 5.

Purity

Purified (same/more than)95% (SDS-PAGE). Endotoxin: (same/less than)1.0 EU/1ug (LAL).

References

1. Leytus, S.P. et al., 1988, Biochemistry 27:1067 - 1074. 2. Tsuji, A. et al., 1991, J. Biol. Chem. 266:16948 - 16953. 3. Li, Y. et al., 2005, Biomed. Biochim. Acta 1681:157 - 165. 4. Kazama, Y. et al., 1995, J. Biol. Chem. 270:66 - 72. 5. Dhanasekaran, S.M. et al., 2001, Nature 412:822 - 826. 6. Wu, Q. and Parry, G. 2007, Front. Biosci. 12:5052 - 5059.

Additional Info

Recognizes human Hepsin.