Hepsin (TMPRSS1)

Hepsin is a type II membrane-associated protein that has an extracellular proteolytic domain and exhibits low sequence homology to other known proteases (1,2). Hepsin overexpression is observed in the prostate, breast, kidney, and ovarian cancers and due to low homology to other known proteases may provide a unique target for pharmacological therapy (3-7). Hepsin is necessary for cell growth in vitro and may play a role in metastatic expansion for Factor VII activation (6,9). The observed molecular mass of hepsin by immunoblotting is 45.3kD. Cloning and characterization of murine and rat hepsin indicate 88% overall homology with human hepsin (10-12). Northern blotting reveals high levels of hepsin transcripts in liver (2,7).\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:200\nImmunohistochemistry: 1-2ug/ml; Formalin-fixed paraffin-embedded sections. Antigen retrieval results in higher background staining and is not recommended.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nK562 cell lysate\n\nStorage and Stability:\nFor long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

H2030-21

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in TBS, pH 7.4, 0.5mg/ml BSA, 0.02% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Tsuji, A., et al., J. Biol. Chem. 266(25): 16,948-16,953 (1991). 2. Leytus, S.P., et al., Biochemistry 27: 1067-1074 (1988). 3. Dhanasekaran, S.M., et al., Nature 412: 822-826 (2001). 4. Magee, J.A., et al., Cancer Res. 61: 5692-5696 (2001). 5. Welsh, J.B., et al., Cancer Res. 61: 5974-5978 (2001). 6. Zacharski, L.R., Thromb. Haemost. 79: 876-877 (1998). 7. Tanimoto, H., et al., Cancer Res. 57: 2884-2887 (1997). 8. Torres-Rosado, A., et al., Proc. Natl. Acad. Sci. USA 90: 7181-7185 (1993). 9. Kazama, Y., et al., J. Biol. Chem. 270(1): 66-72 (1995). 10. Vu, T.-K.H., et al., J. Biol. Chem. 272(50): 31,315-31,320 (1997). 11. Kawamura, S., et al., Eur. J. Biochem. 262: 755-764 (1999). 12. Farley, D., et al., Biochim. Biophys. Acta 1173(3): 350-352 (1993).

Additional Info

Recognizes human hepsin at ~45kD. Species Sequence Homology: Mouse and rat: 88% (17/20aa)