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Heregulin (NDF, GGF, Neuregulin) (BSA & Azide Free)

Cat no: H2030-40X

Heregulin (NDF, GGF, Neuregulin) (BSA & Azide Free)

Heregulin (HRG) or neu differentiation factor (NDF) binds to c-erbB-3 and cerbB-4 receptors on mammary and neuronal cells with low and high affinities, respectively. HRG/NDF exists in several isoforms, which are classified in two groups, \nand that differ in their EGF-like domain and in receptor binding affinity. \n\nApplications:\nSuitable for use in Immunoprecipitation, Western Blot, and Immunohistochemistry. Other applications not tested. \n\nRecommended Dilution: \nImmunoprecipitation: 2ug/mg protein lysate. Use Protein A\nWestern Blot: 1-2ug/ml for 2hrs at RT.\nImmunohistochemistry (Formalin/paraffin): 1:25-1:50 for 60 min at RT. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.\nOptimal dilution determined by the researcher. \n\nPositive Control: \nHRGa1 or HRGb1 recombinant protein. Prostate carcinoma. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H2030-40X

Size

100ul

Applications

IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See H2030-40

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2a

References

1. Lupu, R., et al. (1995) Seminars in Cancer Biology, 6:135

Additional Info

Recognizes rat Heregulin. Does not inhibit the binding of HRG to ErbB receptors. Species Crossreactivity: Human and mouse.

Read more on Supplier website

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

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