Heregulin (Pro-neuregulin-1, Membrane-bound Isoform, Pro-NRG1, Neuregulin-1, Acetylcholine Receptor-inducing Activity, ARIA, Glial Growth Factor, HRG, Neu Differentiation Factor, Sensory and Motor Neuron-derived Factor, Nrg1, Ndf)

The heregulins are a family of ligands that induce phosphorylation of the p185HER-2/neu receptor. Several forms of heregulin (HRG- alpha, HRG-beta-1, HRG-beta-2, HRG-beta-3, HRG-beta- gamma) have been described. Heregulin is a 45kD growth factor with homology to epidermal growth factor. This growth factor binds directly to HER-3 and HER-4 and activates heterodimeric receptor complexes composed of the type I receptor tyrosine kinases, i.e. HER-1, HER-2, HER-3, and HER 4. HER-3 and HER-4 receptors bind to more than a dozen isoforms of the heregulins. Heregulin-alpha is a glycoprotein found first in the conditioned medium of breast carcinoma cell line. Investigators have reported a variety of responses of mouse and human breast and ovarian cells to this family of ligands including growth stimulation, growth inhibition, apoptosis and induction of differentiation in cells expressing the HER-2/neu receptor. A number of studies clearly indicate a growth-stimulatory role for this ligand in human breast and ovarian epithelial cells. In some instances, the breast cancer cells themselves produce heregulin.\n\nApplications:\nSuitable for use in ELISA, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.1-1ug/ml\nWestern Blot: 0.5-2ug/ml\nImmunohistochemistry: 1:100 at RT for 10min\nImmunoprecipitation: 3-5ug/extract from 10e7 cells\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

H2030-41A

Size

50ug

Applications

ELISA, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Guerra-Vladusic F, et al (1998) Ca. Res. 39, 410. 2. Aguilar, Z. et al (1999) Oncogene 18, 6050. 3. Guerra-Vladusic F, et al (1999) Int. J. Oncol. 15, 883. 4. Bagheri-Yarmand, et al (2000) J. Biol. Chem. 275, 39451. 5. Zuleima Aguilar and Dennis J. Slamon (2001) J. Biol. Chem. 276, 44099.

Additional Info

Recognizes human Heregulin.