Herpes Simplex Virus I IgG (HSV I, Herpes Simplex Virus 1, HSV1) BioAssay(TM) ELISA Kit

Sample Type:\nSerum\n\nIntended Use:\nThe HSV-1 IgG ELISA is intended for use in evaluating serologic status to herpes simplex virus (HSV) type 1 infection, or for evaluating paired sera for the presence of a significant increase in HSV-1 specific IgG.\n\nIntroduction:\nHerpes Simplex Virus is a common pathogen and its primary infection is usually asymptomatic. There are two immunologically distinct types of HSV: Type 1 and Type 2. HSV-1 is generally associated with oral infection and lesions above the waist, and HSV-2 is associated with genital infections and lesions below the waist. Clinical cases primarily are (1) eczema herpeticum with eczematous skin changes with numerous lesions, (2) gingivo-stomatitis, and (3) herpes sepsis, almost only found in premature newborn infants. The HSV-1 IgG ELISA is an accurate serologic method to detect HSV-1 IgG specific-antibody in serum samples.\n\nTest Principle:\nPurified HSV-1 antigen is coated on the surface of microwells. Diluted serum is added to the wells, and the HSV-1 IgG specific-antibody, if present, binds to the antigen. All unbound materials are washed away. HRP-conjugate is added, which binds to the antibody-antigen complex. Excess HRP-conjugate is washed off and a solution of TMB Reagent is added. The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount of HSV-1 IgG-specific antibody in the sample. The results are read by a microwell reader compared in a parallel manner with calibrator and controls.\n\nKit Components:\nAntigen-Coated Wells (1 plate, 96 wells), Microtiter Wells coated with purified HSV-1 antigen\nEnzyme Conjugate Reagent (12ml), Contains goat anti-human IgG conjugated to horseradish peroxidase with preservatives\nSample Diluent (22ml), Contains phosphate buffer with preservatives\nNegative Control (150ul/vial), Contains HSV-1 IgG negative serum with preservatives\nCut-off Calibrator (150ul/vial), Contains HSV-1 IgG weak positive serum with preservatives; HSV-1 IgG Index = 1\nPositive Control (150ul/vial), Contains HSV-1IgG positive serum with preservatives\nWash Buffer Concentrate (20X) (50ml), Contains phosphate buffer and Tween 20\nTMB Reagent (11ml), Contains 3, 3

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SPECIFICATIONS

Catalog Number

H2033-13H

Size

1Kit

References

1 The wash procedure is critical. Insufficient washing will result in poor precision and falsely elevated absorbance readings. 2. Serum samples demonstrating gross lipemia, gross hemolysis, or turbidity should not be used with this test. Perspective. New York, Elsevier, 1981: 3-9. Vestergaard, B.F., Grauballe, P.C. and Spanggaard, H., Titration of herpes simplex virus antibodies in human sera by the enzyme-link immunosor\n(section B): 466-468. Coleman, R.M., Pereira, L., Bailey, P.D., et al.,