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HES1 (Hairy Family Helix-loop-helix Transcription Factor)

Cat no: H2034-35

HES1 (Hairy Family Helix-loop-helix Transcription Factor)

HES1 is a transcriptional repressor of genes that require a bHLH protein for their transcription. It may act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. HES1 is expressed in developing neuroectodermal and endodermal endocrine tissues, and HES1 deficient embryos show severe defects in neuronal development, as well as pancreatic hypoplasia.\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry and Immunofluorescence. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot (ECL): 1:200-1:1000 \nImmunohistochemistry (Frozen sections): 1:200-1:1000 \nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHuman neural stem cells, Raji cell lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H2034-35

Size

100ug

Applications

IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.2. No preservative added.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

US Biological Application References: 1. Wang, X., et al., J. Cancer Res. Clin. Oncol. 136: 989-996 (2010). 2. Burkert, J., et al., J. Pathol. 214: 564-573 (2008).

Additional Info

Recognizes human HES1 (Hairy family) at ~29kD. Species Crossreactivity: rodent.

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