HevB1, Hevea brasiliensis, Control Peptide (Rubber Elongation Factor Protein, REF, Hev b 1)

Control Peptide for H2037-01 and H2037-01A, polyclonal antibodies to HevB1.\n\nRubber (cis-1,4-polyisoprene), an isoprenoid polymer, is produced in about 2000 plant species. It is the raw material of choice for gloves, tires and other industrial products. Allergy to natural rubber latex products has been recognized as a serious medical problem especially among health care workers and children with spina bifida. Interestingly, there appears to be significant clinical and immunochemical crossreactivity between some latex proteins and allergens in certain fruits and vegetables, such as banana, kiwi, avocado and potato, and patients with fruit and vegetable allergy may be at increased risk for reacting to latex proteins. Proteins leached from the gloves or extractable latex proteins, ranging in size from 5-200kD, have been shown to be involved in eliciting type I hypersensitivity. Hevea brasiliensis has been the only commercial source of natural rubber mainly because of its abundance in the tree, its quality, and the ease of harvesting. Latex is produced in laticifers, which are specialized structures that consist of anastamosed latexproducing cells. Harvested Hevea latex is a complete cytosol with high protein content. Several potential allergens have been identified in Hevea latex, including rubber elongation factor (REF or Hev b 1), hevamine-1,3-glucanase (Hev b 2), hevein preprotein (HEV1), a 24kD rubber particleassociated protein (RPP) (Hev b 3), and a component of the microhelix protein complex (Hev b 4), a 16kD acidic protein Hev b 5, Hev b 6, and Hev b 7. HevB1 (REF or rubber elongation factor is 138aa protein protein accession #P15252).\n\nSource:\n3 different 15-16aa synthetic peptides from the N-terminus, middle, and C-terminus of Hevb1 proteins conjugated toKLH (#P15252).\n\nMolecular Weight:\n~3kD\n\nApplications:\nSuitable for use in ELISA and Antibody Blocking. Not suitable for Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 50-100ng of control peptide/well\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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