HGF, Recombinant, Human, aa32-728 (Hepatopoietin A, Scatter Factor, SF)

HGF, also known as scatter factor and hepatopoietin A, is a pleiotropic protein in the plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE-like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity (1-5). Human HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide-linked HGF with a 60kD alpha and 30kD beta chain. Alternate splicing generates human HGF isoforms that lack the proteinase-like domain and different numbers of the Kringle domains. Human Pro-HGF shares 90%-93% aa sequence identity with bovine, canine, feline, mouse, and rat Pro-HGF. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET (6, 7). HGF-dependent c-MET activation is implicated in the development of many human cancers (8). HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis (9, 10). HGF induces the up-regulation of Integrin alpha2beta1 in epithelial cells by a selective increase in alpha2 gene transcription (11). This Integrin serves as a Collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis (11, 12). HGF can also alter epithelium morphology by the induction of Nectin-1alpha ectodomain shedding, an adhesion protein component of adherens junctions (13). In the thymus, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH-stimulated iodine uptake (14). HGF promotes the motility of cardiac stem cells in damaged myocardium (15).\n\nSource:\nRecombinant protein corresponding to aa32-728 from human HGF Propeptide.\n\nMolecular Weight: \n~84kD\n\nEndotoxin:\n<0.10EU/1ug (LAL method).\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

145704

Size

10ug

Form

Supplied as a lyophilized powder in PBS. Reconstitute at 100ug/ml PBS.

Purity

~95% (SDS-PAGE)

References

1. Karihaloo, A. et al. (2005) Nephron Exp. Nephrol. 100:e40. 2. Hammond, D.E. et al. (2004) Curr. Top. Microbiol. Immunol. 286:21. 3. Rosario, M. and W. Birchmeier (2004) Dev. Cell 7:3. 4. Lesk, A.M. and W.D. Fordham (1996) J. Mol. Biol. 258:501. 5. Nakamura, T. et al. (1989) Nature 342:440. 6. Mizuno, K., et al. (1994) J. Biol. Chem. 269:1131. 7. Gheradi, E. et al. (2003) Proc. Natl. Acad. Sci. 100:12039. 8. Corso, S. et al. (2005) Trends Mol. Med. 11:284. 9. Maeshima, A. et al. (2000) Kid. Int. 58:1511. 10. Montesano, R. et al. (1991) Cell 67:901. 11. Chiu, S