HIST3H2BB (Histone H2B Type 3-B, H2B Type 12) (FITC)

Histone H2B is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome. Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm. These modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the "histone code" hypothesis). The modifications occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle.\n\nApplications:\nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHeLa cells\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

029496

Size

100Tests

Applications

FC

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.1% sodium azide, 15mg/ml BSA. Labeled with fluorescein isothiocyanate (FITC).

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Qin, L., et al. (2009). Carcinogenesis. 30:1243- 51. 2. Stefan, Z., et al. (2009). Proteomics. 9:521-34. 3. Tatyana, G., et al. (2006). The Journal of biological chemistry. 281:29064-75.

Additional Info

Recognizes human Histone H2B.