Histamine Receptor 1 (HR1)

Histamine, one of the most important mediators of allergy and inflammation, is a chemical messenger and aminergic neurotransmitters. It plays an important role in a multitude of physiological processes in central and peripheral tissues. Histamine is synthesized in a restricted population of neurons located in the tuberomammillary nucleus of the posterior hypothalamus implicated in many brain functions (e.g. sleep/wakefulness, hormonal secretion, cardiovascular control, thermoregulation, food intake, and memory formation). In peripheral tissues histamine is stored in mast cells, basophils, enterochromaffin cells. Histamine release leads to various well-known symptoms of allergic conditions in the skin and the airway system. \n\nApplications:\nSuitable for use in Western Blot and ELISA. Other applications have not been tested.\n\nRecommended Dilutions:\nWestern Blot: 1-10ug/ml using ECL. \nELISA: 1:10,000-1:100,000 using 50-100ng control peptide per well. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H5090

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a liquid in PBS, 0.1% BSA.

P Type

Pab

Purity

Purified by affinity chromatography

Isotype

IgG

References

1. Fujimoto K et al (1993) BBRC, 190:294. 2. Inoue I et al (1996) PNAS 93:13316. 3. DeBacker MD et al (1993) BBRC 197:1601. 4. Fukui H et al (1994) BBRC 201:894. 5. Moguilevsky N et al (1994) Eur. J. Biochem. 224:489.

Additional Info

Recognizes rat Histamine Receptor 1 (HR1). The immunogen shows no significant sequence homology with other histamine receptors. Species sequence homology: mouse and bovine; 100%, human, fish and monkey; 94%, guinea pig; 80%.