Histone, acetylated BioAssay(TM) Sample Kit

Supplier:

United States Biological

Cat no: H5110-05

Histone, acetylated BioAssay(TM) Sample Kit

The Acetyl-Histone Antibody Sampler Kit provides a fast and economical means to evaluate the activation status of the acetylation sites on all of the histones. The kit contains enough primary and secondary antibodies to perform four mini-blot experiments.\n\nModulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin (1). The N-terminal tail of core histones undergoes different posttranslational modifications including acetylation, phosphorylation and methylation (2-4). These modifications occur in response to cell signal stimuli and have a direct effect on gene expression. In most species, the histone H2B is primarily acetylated at lysines 5, 12, 15 and 20 (4,5). Histone H3 is primarily acetylated at lysines 9, 14, 18 and 23 (2,3). Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (6).

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SPECIFICATIONS

Catalog Number

H5110-05

Size

8x40ul

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.

Purity

Purified by protein A and peptide affinity chromatography.

References

Background References: (1) Workman, J.L. and Kingston, R.E. (1998) Annu. Rev. Biochem. 67, 545

Additional Info

Note: Adherence to the attached protocol is extremely important.

Read more on Supplier website

SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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