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Histone, Core

Cat no: H5110-10

Histone, Core

Autoantibodies to histone antigens have been described in patients with idiopathic and drug-induced SLE, rheumatoid arthritis and other conditions. The presence of autoantibodies to histones are frequently found in several rheumatic disorders. In one study, the predominant responses to histones in SLE sera were to H1, H2b, and H3. Marked elevations of binding occurred to H1 and H2b in 33% of patients, while 25% showed higher binding to H3. The same study showed the highest anti-histone reactivity to be in patients with rheumatoid arthritis with vasculitis, while the highest reactivity in SLE sera was in those patients with a history of photosensitivity. In diploid eukaryotic cells, the chromatin fibers are about 20nm in diameter. They consist of two major components in equal amounts, DNA and basic proteins called histones. Histones are believed to be regularly arranged in the deep grove of the DNA helix. The recurring positive charges of the histones form electrostatic associations with the negatively charged phosphate groups of DNA making the DNA more stable and flexible. This allows for the supercoiling of the chromatin fibers.\n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applilcations have not been tested.\n\nRecommended Dilutions:\nImmunoprecipitation: 1:20\nWestern Blot: 1:200\nOptimal dilutions to be determined by the researcher.\n\nMolecular Weights of Histones:\nLysine Rich (H1, f1): ~21,500 \nSlightly Lysine Rich (H2a, f2a2): 14,004 \nSlightly Lysine Rich (H2b, f2b): 13,774 \nArginine Rich (H3, f3): 15,324 \nArginine Rich (H4, f2a1): 11,282\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H5110-10

Size

250ug

Applications

ELISA, IP, WB

Hosts

Sheep

Reactivities

Hum, Bov

Form

Supplied as a liquid in PBS, pH 7.4. No preservatives added.

P Type

Pab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

US Biological application references: 1.Poitras, M.F. et al., (2007) Neuroscience 148:198

Additional Info

Recognizes all bovine histones at ~15kD. Species Crossreactivity: human

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