Histone Deacetylase 1 (HDAC 1)

The histone tail acetylation promotes transcriptional activation. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription (1,2). HAT complexes interact with sequence-specific activator proteins and target specific genes. In addition to histones, some HATs can acetylate nonhistone proteins, suggesting multiple roles for these enzymes (3). In another respect, histone deacetylation is involved in silencing of gene transcription (4). Mammalian histone deacetylases can be divided into two classes on the basis of their similarity to various yeast histone deacetylases (5). The first class is represented by its closeness to the yeast Rpd3- like proteins (HDAC 1, 2, 3 and 8), and the second class, which has similarities to yeast Hda1-like proteins (HDAC 4, 5, 6 and 7).\n\nApplications: \nSuitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution: \nWestern Blot: 1:1000\nImmunoprecipitation: 1:100\nImmunohistochemistry: 1:100 (Paraffin)\nImmunocytochemistry: 1:1000 (ABC detection method).\n\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H5109-46

Size

100ul

Applications

ELISA, ICC, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

P Type

Pab

Purity

Purified by Protein A and immunoaffinity chromatography.

Isotype

IgG

References

1.

Additional Info

Detects endogenous levels of total HDAC 1 protein. It does not crossreact with other HDAC proteins. Species Crossreactivity: Human, monkey, mouse and rat.