Histone H2a, Recombinant, Human (Histone H2A Type 1-A, Histone H2A/r, H2AFR, Histone Cluster 1 H2aa, HIST1H2AA, bA317E16.2)

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.\n\nSource:\nRecombinant corresponding to aa2-130 from human Histone 2A, fused to His tag at N-terminal, expressed in E. coli.\n\nMolecular Weight:\n~14.8kD\n\nApplications:\nSuitable for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

137283

Size

1mg

Form

Supplied as a liquid in 50mM Tris-HCl, pH 8.0, 150mM sodium chloride, 10% glycerol, 1mM PMSF, 1mM DTT.

Purity

Highly Purified (~90%)

References

1. Osley, M.A. Brief Funct. Genomic Proteomic 5(3):179-89 (2006). 2. Wyrick, J.J. and Parra, M.A.. Biochim Biophys Acta. 1789(1):37-44 (2009). 3. Zhou, W., et al. Int J Biochem Cell Biol. 41(1):12-5 (2009).