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Histone H2A.X, phosphorylated (Ser139) (H2A Histone Family Member X, H2A.FX, H2A/X, H2AFX, H2AX)

Cat no: H5110-03K1

Histone H2A.X, phosphorylated (Ser139) (H2A Histone Family Member X, H2A.FX, H2A/X, H2AFX, H2AX)

As a member of the histone H2A family, histone H2A.x (H2A.x) is a variant histone H2A which replaces conventional H2A in a subset of nucleosomes. H2A.x is involved in the DNA repair of double-strand breakage (DSB) damage on nuclear DNA. After a double strand DNA break, H2A.x is rapidly phosphorylated at serine 139 by ATM kinase and becomes gamma-H2AFX. This phosphorylation step can extend up to several thousand nucleosomes from the actual site of the DSB and may mark the surrounding chromatin for recruitment of proteins required for DNA damage signaling and repair (1). As a part of post-translational modifications during apoptosis caused by severe DNA damage, high expression of phosphorylated H2A.x is considered as an accurate indicator of apoptosis (2)\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot (Human, mouse, rat): 1:1000-1:10,000\nImmunohistochemistry (Human, mouse): 1:50-1:100\nImmunocytochemistry (Human, mouse): 1:50-1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H5110-03K1

Size

100ul

Applications

ICC, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Crowe, S, et al; Eur J Neurosci. 23(9): 2351 2. Song, X, et al; Mol Cell Biol. 27(7): 2648

Additional Info

Recognizes human Histone H2A.X at ~14kD when phosphorylated at Ser139. Species Crossreactivity: Rat, mouse

Read more on Supplier website

Applications

FC

Hosts

Mouse

Reactivities

Hum

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Applications

FC

Hosts

Mouse

Reactivities

Hum

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Applications

FC

Hosts

Mouse

Reactivities

Hum

More info

Applications

FC

Hosts

Mouse

Reactivities

Hum

More info

Applications

FC

Hosts

Mouse

Reactivities

Hum

More info

Applications

FC

Hosts

Mouse

Reactivities

Hum

More info

Applications

FC

Hosts

Mouse

Reactivities

Hum

More info

Applications

FC

Hosts

Mouse

Reactivities

Hum

More info
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