Histone H3, acetylated (Lys9)

Applications: \nSuitable for use in Western Blot, Chromatin IP and Beadlyte Histone-Peptide Specificity Assay. Other applications not tested. \n\nRecommended Dilution:\nImmunoblot Analysis: A 1:5000-1:10,000 dilution of this lot detected acetyl-Histone H3 (Lys9) in acid extracts from sodium butyrate treated HeLa cells.\nChromatin Immunoprecipitation (ChIP): An independent laboratory has shown this antibody preferentially immunoprecipitates chromatin from wild type yeast and not from a yeast strain containing a Lysine substitution to Alanine at residue 9.\nBeadlyte Histone-Peptide Specificity Assay: A 1:1000-1:5000 dilution of this lot was incubated with histone H3 peptides containing various modifications conjugated to Luminex(R) microspheres. No crossreactivity with peptides containing acetyl-lysine 14 or acetyl-lysine 27 was observed.\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H5110-13A

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid containing 0.05% sodium azide before the addition of glycerol to 30%.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Suka, N., et al., Mol. Cell 8: 473, 2001

Additional Info

Recognizes acetyl-histone H3 (Lys9), Mr 17kD. An additional unknown protein was detected at Mr 23kD. Species Crossreactivity: Yeast and human. Broad species crossreactivity is expected.