Histone H3, acetylated (Lys9)

Cellular Localization: Nuclear\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H5110-13G4

Size

50ul

Applications

ELISA, ICC, WB

Hosts

Mouse

Reactivities

Hum

Form

0.01M PBS. pH 7.4

P Type

Mab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG1

References

Fry CJ & Peterson CL Transcription. Unlocking the gates to gene expression. Science 295:1847-8 (2002). <PUBMED:11884741>Strahl BD & Allis CD The language of covalent histone modifications. Nature 403:41-5 (2000). <PUBMED:10638745>Cheung P et al. Signaling to chromatin through histone modifications. Cell 103:263-71 (2000). <PUBMED:11057899>Krebs JE et al. Global role for chromatin remodeling enzymes in mitotic gene expression. Cell 102:587-98 (2000). <PUBMED:11007477>Kornberg RD & Lorch Y Twenty-five years of the nucleosome, fundamental particle of the eukaryote chromosome. Cell 98:285-94 (1999). <PUBMED:10458604>Luger K & Richmond TJ The histone tails of the nucleosome. Curr Opin Genet Dev 8:140-6 (1998). <PUBMED:9610403>Struhl K Histone acetylation and transcriptional regulatory mechanisms. Genes Dev 12:599-606 (1998). <PUBMED:9499396>Zhang W et al. Essential and redundant functions of histone acetylation revealed by mutation of target lysines and loss of the Gcn5p acetyltransferase. EMBO J 17:3155-67 (1998). <PUBMED:9606197>Kuo MH et al. Transcription-linked acetylation by Gcn5p of histones H3 and H4 at specific lysines. Nature 383:269-72 (1996). <PUBMED:8805705>Sobel RE et al. Conservation of deposition-related acetylation sites in newly synthesized histones H3 and H4. Proc Natl Acad Sci U S A 92:1237-41 (1995). <PUBMED:7862667>Turner BM & O'Neill LP Histone acetylation in chromatin and chromosomes. Semin Cell Biol 6:229-36 (1995). <PUBMED:8562915>Thorne AW et al. Patterns of histone acetylation. Eur J Biochem 193:701-13 (1990). <PUBMED:2249688>

Additional Info

Staining of (acetyl K9) histone H3 in immunoblotting is specifically inhibited with the immunizing peptide. There is no inhibition with non-acetylated histone H3 peptide. Species Crossreactivity: Crossreacts with Human. Expected to crossreact with Mouse, Rat, Xenopus laevis, bovine, a wide range of other species, C. elegans, Chicken and Drosophilia melanogaster due to very high or perfect sequence identity across many species.

Alternative Names

H3, HisH3, His1H3, H3/a, H3/b, H3/c, H3/d, H3/f, H3/h, H3/i, H3/j, H3/k, H3/l