Histone H3, acetylated (Lys9)

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Acetylation of histone H3 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs). Acetylation of lysine14 is commonly seen in genes that are being actively transcribed into RNA.\n\nApplications: \nSuitable for use in Western and Dot Blot. Other applications not tested.\n\nRecommended Dilutions: \nWestern Blot: 1:500. Detects acetylated histone H3 on 10ug of HEK293 lysates.\nDot Blot: 0.5ug/ml. Detects 50ng of mono-acetylated peptide corresponding to position Lys9 in the N-terminal sequence of Histone H3. Does not detect the mono-acetylated peptide corresponding to acetyl-lysine at position 14 or unacetylated Histone H3.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nTSA-treated HeLa and NIH/3T3 cells.\n\nRecommended Secondary Antibodies:\nI1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb\nI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb\nI1904-46Q: IgG, H&L (HRP) Pab Gt x Rb\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H5110-14A

Size

200ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Su, R.C., et al., Nat. Genet. 36: 502-506 (2004). 2. Li, J., et al., J. Biol. Chem. 278: 1183-1191 (2003). 3. Bjerling, P., et al., Mol. Cell Biol. 22: 2170-2181 (2002). 4. Kruhlak, M., et al., J. Biol. Chem. 276: 38,307-38,319 (2001). 5. Nowak, S. & Corces, V., Genes Dev. 14: 3003-3013 (2000). 6. Salvador, L.M., et al., J. Biol. Chem. 276: 40,146-40,155 (2001). 7. Zhong, S., et al., J. Biol. Chem. 276: 33,213-33,219 (2001).

Additional Info

Recognizes human Histone H3 (Mr 17kD) acetylated at position Lys 9. Species Crossreactivity: mouse, rat and Eukaryote.