Histone H3, Dimethyl (Lys9)

Applications: \nSuitable for use in Western Blot, Peptide Inhibition and Beadlyte(R) assay. Other applications not tested. \n\nRecommended Dilution:\nImmunoblot Analysis: A 1:1000-1:5000 dilution of this lot detected methylated Histone H3 in acid-extracted proteins from HeLa cells. The antibody did not detect unmethylated recombinant Histone H3.\nPeptide Inhibition: Specificity of this lot was confirmed by the ability of 1uM of the immunizing peptide to completely abolish detection of Histone H3 in immunoblot analysis of HeLa acid extracts. No competition was observed with peptides containing monomethyl-lysine 9, trimethyl-lysine 9, monomethyl-lysine 27, dimethyl-lysine 27, or trimethyl-lysine 27.\nBeadlyte(R) Histone-Peptide Specificity Assay: A 1:1000 dilution of this lot was incubated with histone H3 peptides containing various modifications conjugated to Luminex? microspheres.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H5110-14E3

Size

200ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid containing 0.05% and 30% glycerol.

P Type

Pab

Purity

Serum depleted of non-specific Histone H3 antibodies

Isotype

IgG

References

1. Nakayama, J. I., et al., Science 292: 110-113, 2001.

Additional Info

Recognizes dimethylated Histone H3 (Lys9), Mr 17kD with a nonspecific protein detected at 97kD. Species Crossreactivity: Human. A broad species crossreactivity is expected.