Histone H3

Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).\n\nApplications:\nSuitable for use in Western Blot. Other applications have not been tested.\n\nRecommended Dilution: \nWestern Blot: 1:1000 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H5110-13D

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov, NHP, Prc

Form

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added. Also available without BSA & Glycerol. See H5110-13DX.

P Type

Pab

Purity

Purified by Protein A affinity chromatography and immunoaffinity chromatography.

Isotype

IgG

References

1. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79. 2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41. 3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5. 4. Cheung, P. et al. (2000) Cell 103, 263-71. 5. Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73. 6. Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.\n7. Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13. 8. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60. 9. Goto, H. et al. (1999) J Biol Chem 274, 25543-9. 10. Preuss, U. et al. (2003) Nucleic Acids Res 31, 878-85. 11. Dai, J. et al. (2005) Genes Dev 19, 472-88. 12. Biochim Biophys Acta. 2008 Aug 13.Hoover LL, Burton EG, O'Neill ML, Brooks BA, Sreedharan S, Dawson NA, Kubalak SW

Additional Info

Recognizes endogenous levels of total human histone H3 protein. Does not recognize other histones. Species Cross-Reactivity: mouse, rat, monkey, bovine, porcine and zebrafish. Species sequence homology: D. melanogaster.\n\n