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Histone H3

Cat no: H5110-24

Histone H3

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Histone H3 is of length 135aa and is essential for the formation of the highly ordered chromatin structure that has been maintained throughout evolution. H3 histone is from the central building block of the nucleosomal core. It is wrapped around a nucleosome composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). Histone modifications and variants have key roles in the activation and silencing of genes. Phosphorylation of histone H3 at serine 10 and serine 28 is involved in transcriptional activation of genes responding to stress or mitogen-stimulated signaling pathways. Histones contribute to virtually all chromosomal processes, such as gene regulation, chromosome condensation, recombination, and replication.\n\nApplications:\nSuitable for use in ELISA. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:100-1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H5110-24

Size

100ul

Applications

ELISA

Hosts

Rabbit

Reactivities

Hum, Bov

Form

Supplied as a liquid, 0.025% sodium azide, 50% glycerol.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Albig, W. et al. Hum. Genet. 97:486-491 (1996). 2. Albig, W. et al. Hum. Genet. 101:284-294 (1997).

Additional Info

Recognizes human Histone H3. Species Crossreactivity: bovine, equine.

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