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HIV-1 env gp120, Oligomeric, Strain IIIB, Recombinant (Human Immunodeficiency Virus-1)

Cat no: H6003-07D

HIV-1 env gp120, Oligomeric, Strain IIIB, Recombinant (Human Immunodeficiency Virus-1)

Full length HIV-1 IIIB envelope gp120 cloned genes expressing oligomeric glycoprotein produced in the Baculovirus Expression System. \n\nSpecificity: \nThis protein binds to murine monoclonal antibodies of defined epitope specificity and HIV-1 converted human serum polyclonal antibodies in ELISA and Western ELISA. \n\nBiological Activity: \nThis protein binds to human T-cell receptor CD4 in ELISA and Western ELISA as determined by CD4/gp120/Anti-gp120 mAb-peroxidase capture ELISA. This protein activates human T-Lymphocytes (CD4+, CD4-), in vitro, as measured by RNA synthesis during G0 to G1 transition phase of antigen-binding competent cells. \n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested. \n\nRecommended Dilutions:\nApplications require 10-100ng protein depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 10-100ng of immobilized protein under standard ELISA conditions.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and freeze at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

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SPECIFICATIONS

Catalog Number

H6003-07D

Size

50ug

Form

Supplied as a liquid in PBS, pH 7.2. No stabilizers or preservatives added.

Purity

Purified by immunoaffinity chromatography to (same/more than) 98% purity as determined by SDS-PAGE, non-reduced and reduced.

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