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HIV-1 gag, p24, Strain IIIB, Recombinant (Human Immunodeficiency Virus-1) (Biotin)

Cat no: H6003-20E2

HIV-1 gag, p24, Strain IIIB, Recombinant (Human Immunodeficiency Virus-1) (Biotin)

Coupling Ratio: \n~10-20 moles of biotin per mole p24. \n\nSpecificity: \nBiotin p24 binds strepavidin-AP/HRP in ELISA and Western ELISA. \n\nBiological Activity: \nBiotin p24 binds to solid phase anti-p24 antibody (oligoclonal) as determined by capture ELISA using strepavidin-HRP as a secondary reagent.\n\nApplications: \nA dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. 1. ELISA may be performed in 1 : 5000 dilution range depending on the concentration of the test ligand and specificity and sensitivity of the secondary reagents. p24/Anti-p24 antibody capture ELISA may be performed in 1-10ng antibody in solid phase and 0.1-1ng of biotin-p24.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.

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SPECIFICATIONS

Catalog Number

H6003-20E2

Size

50ug

Conjugates

Biotin

Form

Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.02% sodium azide. Conjugated to Biotin.

Purity

Purified by cation/anion affinity and gel exclusion chromatography to 98% purity as determined by SDS-PAGE, reduced.

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Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info
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