HIV-1 gp41 (Human Immunodeficiency Virus-1)

Human immunodeficiency virus (HIV) is a retrovirusthat can lead to a condition in which the immune systembegins to fail, leading to opportunistic infections. HIV primarily infects vital cells in the humanimmune systemsuch as helper T cells(specifically CD4+ T cells), macrophagesand dendritic cells. HIV infection leads to low levels of CD4+ T cells through three main mechanisms: firstly, direct viral killing of infected cells; secondly, increased rates of apoptosisin infected cells; and thirdly, killing of infected CD4+ T cells by CD8 cytotoxic lymphocytesthat recognize infected cells. When CD4+ T cell numbers decline below a critical level, cell-mediated immunityis lost, and the body becomes progressively more susceptible to opportunistic infections. HIV was classified as a member of the genus Lentivirus, part of the family of Retroviridae. Lentiviruses have many common morphologies and biological properties. Many species are infected by lentiviruses, which are characteristically responsible for long-duration illnesses with a long incubation period. Lentiviruses are transmitted as single-stranded, positive-sense, enveloped RNA viruses. Upon entry of the target cell, the viral RNA genomeis converted to double-stranded DNAby a virally encoded reverse transcriptasethat is present in the virus particle. This viral DNA is then integrated into the cellular DNA by a virally encoded integraseso that the genome can be transcribed. Once the virus has infected the cell, two pathways are possible: either the virus becomes latentand the infected cell continues to function, or the virus becomes active and replicates, and a large number of virus particles are liberated that can then infect other cells.\n\nApplications:\nSuitable for use in ELISA. Other applications not tested.\n\nRecommended Dilution:\nELISA: Generates 1 OD (410nm) at a dilution of 1: 250 on AS 1001 (DEV-1) ELISA. Optimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

H6003-02A

Size

1ml

Applications

ELISA

Hosts

Rabbit

Form

Supplied as a liquid.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Miller JD, et al., al., J Physiol 2005 Jan 13; 2. Deheyn DD, et al., Mar Environ Res 2005 Jul;60(1):1-33 3. Sosnowski M, et al., Ann Noninvasive Electrocardiol 2005 Jan;10(1):7-15 4. Zinkstok RT, et al., Opt Lett 2005 Jan 1;30(1):78-80 5. Schlatter A, et al., Opt Lett 2005 Jan 1;30(1):44-6 6. Multidimensional, mapping-based complex wavelet transforms. Fernandes FC, van Spaendonck RL, IEEE Trans Image Process 2005 Jan;14(1):110-24.

Additional Info

Recognizes HIV-I gp41. Generates a Strong positive control spot on HIVSav 1+2.