HIV-1, p24 (Human Immunodeficiency Virus 1), ELISA Kit, BioAssay(TM)

Supplier:

United States Biological

Cat no: H6005-10P

HIV-1, p24 (Human Immunodeficiency Virus 1), ELISA Kit, BioAssay(TM)

Principle of the Assay:\nMicrotitration wells coated with murine anti-HIV-1 P24 capture antibody, are exposed to test specimens, which may contain p24 reactive determinants. After an incubation period, unbound components in the test sample are washed away. Specifically bound p24 reactive determinants react with a mouse anti-HIV-1 p24 conjugated with biotin during a second incubation period. Following a second wash cycle the biotinylated antibody is detected by the addition of a streptavidin HRP conjugate. Following a third wash cycle, specifically bound enzyme conjugate is detected by reaction with the Substrate solution, tetramethylbenzidine (TMB). The assay is measured spectrophotometrically to indicate the level of p24 reactive determinants present in a sample.\n\nQualitative Analysis:\nNegative control should be (same/less than)0.10\n100pg/ml control should be (same/more than)0.60\n\nSensitivity: \n1.7pg/ml.\n\nKit Components:\nH6005-10P1: Microtiter Strips, 1x96 wells \nPlastic microtitration wells coated with anti-HIV-1 p24 murine monoclonal antibody in foil pouch with desiccant. \nH6005-10P2: Positive Control 10ng/ml, 1x100ul\nH6005-10P3: Lysis Buffer, 1x6ml\n*H6005-10P4: Antibody (Biotin), 1x12ml\nH6005-10P5: Streptavidin (HRP), 1x12ml \nContaining 0.01% Bromonitrodioxane as preservative. \nH6005-10P6: Wash Buffer 20x, 2x60ml \nTris buffered saline pH 7.8-8.0, containing 0.05% Tween 20. Must be diluted before use.\nH6005-10P7: Substrate (TMB) Solution, 1x12ml \nReady to use. \nH6005-10P8: Stop Solution. 1N H2SO4, 1x12ml\n\nStorage and Stability:\nAliquot and store *H6005-10P4 at -20 degrees C. Store other components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H6005-10P

Size

1Kit

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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