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HIV-1 p66, Strain IIIB, Recombinant (Reverse Transcriptase, Human Immunodeficiency Virus-1)

Cat no: H6003-15H

HIV-1 p66, Strain IIIB, Recombinant (Reverse Transcriptase, Human Immunodeficiency Virus-1)

Full length Recombinant heterodimeric HIV-1 IIIB p66 (RT) produced in E.coli expression system. \n\nSpecificity: \nThis protein shows reactivity with murine monoclonal antibodies and human polyclonal antibodies in ELISA and Western ELISA. \n\nBiological Activity: \n0.1 unit of purified RT incorporates a minimum of 5 picomoles of dTTP per minute under standard RT assay conditions. \n\nApplications: \nA dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 1-10ng protein depending on the nature and affinity of the test antibody. Solid phase antigen in 50-100ng amounts shows reactivity with human serum antibodies in Dot Blot assays\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.

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SPECIFICATIONS

Catalog Number

H6003-15H

Size

50ug

Form

Supplied as a liquid in 50mM Tris, pH 8.0, 10% glycerol, 1mM DTT, 0.2M sodium chloride. No stabilizers or preservatives added.

Purity

Purified by ion-affinity chromatography to (same/more than) 90% purity as determined by SDS-PAGE, reduced.

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