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HIV-1 tat, Strain IIIB, Recombinant (Human Immunodeficiency Virus-1) (Gold)

Cat no: H6007-10D

HIV-1 tat, Strain IIIB, Recombinant (Human Immunodeficiency Virus-1) (Gold)

tat HIV-1 IIIB conjugated to Colloidal Gold (25nm mean particle size). \n\nCoupling Ratio: \nOD520nm = 10; protein content approximately 25ug. \n\nSpecificity: \nGold conjugated tat binds to murine monoclonal antibodies of defined epitope specificity and human/rabbit polyclonal antibodies as determined by dot blot assays.\n\nApplications: \nA dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. 1. Dot blot assays may be performed in 1:100 dilution range depending on the concentration of solid phase Anti-tat in the test system. 2. Immunohistochemical studies may be performed in 1:50 dilution range.\n\nStorage and Stability:\nMay be stored at 4 degrees C. For long-term storage, aliquot and store at 4 degrees C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Gold conjugates are light sensitive.

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SPECIFICATIONS

Catalog Number

H6007-10D

Size

1ml

Conjugates

Gold

Form

Supplied as a liquid in 10mM Tris, pH 8.0, 5% BSA, 0.05% PEG, 0.02% sodium azide. Conjugated to Colloidal Gold.

Purity

Purified by ion affinity and reverse phase HPLC to (same/more than) 99% as determined by SDS-PAGE and HPLC.

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