HLA-A(ID) (HLA-A, HLAA, HLA class I histocompatibility antigen, A-3 alpha chain, MHC class I antigen A*3) (APC)

HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described.\n\nApplications:\nSuitable for use in Western Blot, ELISA.\n\nRecommended Dilution:\nELISA: 1:1000\nWestern Blot: 1:100~250\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C with glycerol. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Do not freeze APC conjugates. Light sensitive.

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SPECIFICATIONS

Catalog Number

031022-APC

Size

100ul

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Mouse

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Allophycocyanin (APC).

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

HLA Class I and Genetic Susceptibility to Type 1 Diabetes: Results from the Type 1 Diabetes Genetics Consortium. Noble JA, et al. Diabetes, 2010 Aug 26. PMID 20798335. Effect of HLA Class I and Class II Alleles on Progression from Autoantibody Positivity to Overt Type 1 Diabetes in Children with Risk-Associated Class II Genotypes. Lipponen K, et al. Diabetes, 2010 Aug 25. PMID 20739684. Infectious mononucleosis-linked HLA class I single nucleotide polymorphism is associated with multiple sclerosis. Jafari N, et al. Mult Scler, 2010 Aug 24. PMID 20736246. [Associations of Human Leukocyte Antigen-A, B, DRB1 Genes with Leukemia Patients in Anhui Province of China.] Liao YQ, et al. Zhongguo Shi Yan Xue Ye Xue Za Zhi, 2010 Jul. PMID 20723328. Longer survival associated with HLA-A*03, B*14 among 212 hemochromatosis probands with HFE C282Y homozygosity and HLA-A and -B typing and haplotyping. Barton JC, et al. Eur J Haematol, 2010 Aug 14. PMID 20722701.

Additional Info

Mouse

Alternative Names

HLA-A, HLAA, HLA class I histocompatibility antigen, A-3 alpha chain, MHC class I antigen A*3