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HLA Class 2 Antigen DP

Cat no: H6099-84B

HLA Class 2 Antigen DP

HLA-DPA1 belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta (DPB) chain, both anchored in the membrane. It plays a vital role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (B lymphocytes, dendritic cells and macrophages). Within the DP molecule the alpha and beta chain contain the polymorphisms governing the peptide binding specificities, resulting in up to 4 different molecules. Can be used for tissue typing on lymphoblastoid cells, transplantation typing, disease susceptibility and detection of class II products.\n\nApplications: \nSuitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 1:100-1:2000\nWestern Blot: 1:100:1:2000\nImmunohistochemistry (Frozen): 1:10-1:2000\nFlow Cytometry: 1:10-1:1000\nImmunoprecipitation: 1:10-1:500\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

H6099-84B

Size

100ug

Applications

ELISA, FC, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2. No preservative added.

P Type

Mab

Purity

Purified

Isotype

IgG2a

References

1. Heyes J et al. Monoclonal antibodies to HLA-DP-transfected mouse L cells. Proc Natl Acad Sci U S A 83:3417-21 (1986).

Additional Info

Recognizes human HLA Class 2 Antigen DP.

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