HSF1

Detects an ~85kDa protein in unstressed cell lysates, and an ~95kDa protein in heat shocked cell lysates, corresponding to the molecular mass of inactive and active forms of HSF1 on SDS PAGE immunoblots.

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SPECIFICATIONS

Catalog Number

SMC-118D

Size

100ug

Applications

ELISA, ICC, IP, WB

Hosts

Rat

Reactivities

Hum, Mouse, Rat, Bov, GP, Ham, Rabbit

Form

Protein G Purified

Clone

10H8

Gene Id

3297

Isotype

IgG1

Accession

NP_005517.1

Dilutions

1ug/ml was sufficient for detection of HSF1 by ECL immunoblot in 20ug of HeLa lysate.

References

1.Cotto J.J., Fox S.G. and Morimoto R.I. (1997) J. Cell Science 110: 2925-2934.\n2.Morano K.A. and Thiele D.J. (1999). Gene Expression 7\n(6): 271-82.\n3.Tanaka KI et al. (2007). JBC Papers Online Manuscript\nM704081200.\n4.Morimoto R. I. (1998) Genes Dev 12:

Swiss Prot

Q00613

Storage Temp

-20

Concentration

1mg/mL

Research Area

Heat Shock

Shipping Temp

Blue Ice or 4

Storage Buffer

PBS pH7.4, 50% glycerol

Alternative Names

HSTF1

Scientific Background

HSF1, or heat shock factor 1, belongs to a family of Heat Shock transcription factors that activate the\ntranscription of genes encoding products required for\nprotein folding, processing, targeting, degradation, and\nfunction (2). The up-regulation of HSP (

Certificate Of Analysis

1 ug/mL of SMC-118 was sufficient for detection of HSF1 in 20ug of heat shocked HeLa cell lysate by ECL immunoblot analysis using Goat anti-rat IgG: HRP as the secondary antibody