Hsp70

Detects a ~70kDa protein corresponding to the molecular mass of inducible Hsp70 on SDS PAGE immunoblots. The mapped epitope is in the region of amino acid residues 436-503. Does not cross-react with Hsc70 (Hsp73).

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SPECIFICATIONS

Catalog Number

SMC-100A

Size

50ug

Applications

ELISA, ICC, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Can, Ch/Bird, GP, Ham, Prc, Rabbit, Sheep

Form

Protein G Purified

Clone

C92F3A-5

Gene Id

3303

Isotype

IgG

Accession

NP_005336.3

Dilutions

1ug/ml was sufficient for detection of Hsp70 in 20uof Hela cell lysate

References

1. Welch W.J. and Suhan J.P. (1986) J Cell Biol. 103: 2035-2050.\n2. Boorstein W. R., Ziegelhoffer T. & Craig E. A. (1993) J.\nMol. Evol. 38(1): 1-17.\n3. Rothman J. (1989) Cell 59: 591-601.\n4. DeLuca-Flaherty et al. (1990) Cell 62: 875-887.\n5. Bork P., Sand

Swiss Prot

P08107

Storage Temp

-20

Concentration

1mg/mL

Research Area

Heat Shock

Shipping Temp

Blue Ice or 4

Storage Buffer

PBS pH7.2, 50% glycerol

Alternative Names

Hsp70 1, Hsp70 2, Hsp70.1, Hsp72, HSPA1, HSPA1A, HSPA1B

Scientific Background

Hsp70 genes encode abundant heat-inducible 70-kDa hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic r

Certificate Of Analysis

1 ug/mL of SMC-100 was sufficient for detection of Hsp70 in 20ug of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.