HSPB8 (Heat Shock Protein beta-8, HspB8, Alpha-crystallin C Chain, H11, CRYAC, HSP22, DHMN2, CMT2L, Heat Shock 22kD Protein 8)

Hsp22 (HSPB8) is a 196aa protein that is a member of the small heat shock protein super-family and the human protein is most closely related to Hsp27. Similar to most other sHSPs, Hsp22 is predominately transcribed in skeletal muscle and heart, as well as the placenta (1). Hsp22 is a monomeric protein which interacts with HSPB1. It displays temperature-dependent chaperone activity. In a two hybrid screen, HspB8 interacted preferentially with a triple aspartate form of Hsp27 which mimics Hsp27 phosphorylated at Ser15, Ser78, and Ser82, as compared to wild-type Hsp27 (2). HSPB8 has two binding domains (N and C Terminal) that are specific for different binding partners, and has the ability to bind itself and other sHSPs (3). The chaperone-like activity is of great importance to the function of Hsp22 in various processes including proliferation, apoptosis and macroautophagy (4). Mutations in the HSPB8 gene are associated with the inherited peripheral neurpathies, autosomal dominant distal hereditary motor neuropathy type IIA (dSMA) and axonal Charcot-Marie-Tooth disease type 2L (CMT2L) (5).\n\nApplications: \nSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

136798

Size

25ul

Applications

IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid.

P Type

Pab

Purity

Supernatant

References

1. Kappe G., et al. (2001) Biochem Biophys Acta 1520: 1- 6. 2. Benndorf R., et al. (2001) J Biol Chem 276: 26753- 26761. 3. Sun X., et al. (2004) J Biol Chem 279: 2394-2402. 4. Kim M.V., et al. (2004) Biochem Biophys Res Commun 325: 649-652. 5. Wilhelmus M.M., et al. (2006)Acta Neuropathol (Berl) 111: 139-149.

Additional Info

Recognizes human Hsp22 at ~22kD. Species Crossreactivity: Mouse and rat. Does not cross-react with Hsp27 or alpha-crystallin.

Alternative Names

Heat Shock Protein beta-8, HspB8, Alpha-crystallin C Chain, H11, CRYAC, HSP22, DHMN2, CMT2L, Heat Shock 22kD Protein 8