HSPE1 (10kD Heat Shock Protein 1, 10kD Chaperonin, Chaperonin 10, CPN10, GROES, EPF)

Chaperonin 10, otherwise known as Cpn10, (groES in E.coli) make up a family of small heart shock proteins with an approximate molecular mass of 10kD (Hsp10s). Cpn10 acts as a co-chaperone and interacts with the Hsp60 family to promote proper folding of polypeptides. Cpn10 and Cpn60 both exhibit sevenfold axis of symmetry and function as a team in the protein folding and assembly process (1). Cpn10 has been located in human platelets, but is also present in human maternal serum (2, 3). It has been reported that human Cpn10 is identical with early pregnancy factor, which is involved in control over cell growth and development. This identification suggest that Cpn10 may act like a hormone in stressful situations such as pregnancy (4).\n\nApplications: \nSuitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

136800

Size

25ug

Applications

ELISA, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov, Can, GP, Prc, Rabbit, Sheep, Xen/Amph

Form

Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

References

1. Velez-Granell C.S., et al. (1994) J of Cell Science. 107(3): 539-549. 2. Morton H., Hegh V., and Clunie G.J.A. (1974) Nature (London) 249: 459-460. 3. Cavanagh A.C., and Morton H. (1994) Eur. J. Biochem. 222: 551-560. 4. Minto M., et al. (1998) Molecular Cell Research. 1403 (2): 151-157.

Additional Info

Recognizes human Anti- Cpn10 at ~10kD. Species Crossreactivity: Mouse, rat, bovine, canine, guinea pig, porcine, rabbit, sheep and xenopus.

Alternative Names

10kD Heat Shock Protein 1, 10kD Chaperonin, Chaperonin 10, CPN10, GROES, EPF