HTR3A (5-hydroxytryptamine receptor 3)

HTR3A (5-HYDROXYTRYPTAMINE RECEPTOR 3A), also called SEROTONIN 5-HT-3 RECEPTOR, is a member of the ligand-gated ion channel receptor superfamily. HTR3A is a neurotransmitter in the central and peripheral nervous systems that plays a role in many physiologic processes such as sleep, appetite, thermoregulation, pain perception, hormone secretion, and sexual behavior. Using fluorescence in situ hybridization, the HTR3 gene is assigned to 11q23.1-q23.2. The single-channel conductance for homomers of HTR3A is 0.4 pS. Replacement of 3 arginine residues unique to the HA-stretch of the HTR3A subunit by their HTR3B subunit counterparts increases single-channel conductance 28-fold. Ultrastructural studies of the Torpedo nicotinic acetylcholine receptor indicated that the key residues might frame narrow openings that contribute to the permeation pathway. The genomic structure of the HTR3A gene contains 7 exons and spans approximately 14.5 kb.\n\nUniProt Number:\nP46098\n\nGene ID:\nHTR3A\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry (Paraffin).\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Handling:\nStore at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term.\nAvoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

144709

Size

100ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1.Bruss, M., Eucker, T., Gothert, M., Bonisch, H. Exon-intron organization of the human 5-HT-3A receptor gene. Neuropharmacology 39: 308-315, 2000. \n2.Kelley, S. P., Dunlop, J. I., Kirkness, E. F., Lambert, J. J., Peters, J. A. A cytoplasmic region determines single-channel conductance in 5-HT(3) receptors. Nature 424: 321-324, 2003.\n3.Maricq, A. V., Peterson, A. S., Brake, A. J., Myers, R. M., Julius, D. Primary structure and functional expression of the 5HT-3 receptor, a serotonin-gated ion channel. Science 254: 432-437, 1991.\n

Additional Info

Recognizes human HTR3A. No crossreactivity with other proteins.