The kit performance has been optimized for the protocol and materials listed below usingstandard dilutions of human Complement C3 in the 0.7 - 500 ng/ml range. The operator must determineappropriate dilutions of reagents for alternative assay conditions. ELISA assay reactivity is sensitive tovariations in operator, pipetting and washing techniques, incubation time, temperature, composition ofreagents, and other experimental variables. Assay optimization may be required to generate the standardcurve and fit the samples in the specified detection range.