Human CTRP3 ELISA employs the quantitatively competitive enzyme immunoassay technique in which human CTRP3 present in samples competed with a fixed amount of biotinylated human CTRP3 for sites on purified rabbit IgG specific against human CTRP3. During the incubation, the rabbit IgG becomes bound to the goat anti-rabbit IgG pre- coated onto the microplates. Following a wash to remove any unbound antibody, standard, samples and biotin conjugate, a Streptavidin conjugated to horseradish-peroxidase (HRP) is added to the wells. After washing away any unbound enzyme, a substrate solution is added to the wells. The enzyme reaction yields a blue product that turns yellow when the Stop Solution is added. The intensity of the color measured is in proportion to the amount of human CTRP3 bound in the initial step. The sample values are then read off the standard curve. Human CTRP3 ELSA has been shown to accurately quantitate the recombinant and natural human CTRP3. Results obtained using natural human CTRP3 showed dose response curves that were parallel to the standard curves obtained using the kit standards.
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