Human GPIIb/IIIa ELISA kit is designed for detection of human GPIIb/IIIa in platelets, platelet-rich plasma and cell culture lysate. This assay employs a quantitative sandwich enzyme immunoassay technique that measures GPIIb/IIIa in less than 4 hours. A polyclonal antibody specific for human GPIIb/IIIa has been pre-coated onto a 96-well microplate with removable strips. GPIIb/IIIa in standards and samples is sandwiched by the immobilized antibody and the biotinylated polyclonal antibody specific for GPIIb/IIIa, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.