The LH Quantitative Test is based on a solid phase enzyme-linked immunosorbent assay (ELISA). The assay system utilizes a mouse monoclonalanti-alpha-LH antibody for solid phase (microtiter wells) immobilizationand a mouse monoclonal anti-β-LH antibody in the antibody-enzyme(horseradish peroxidase) conjugate solution. The test sample is allowed toreact simultaneously with the antibodies, resulting in LH molecules beingsandwiched between the solid phase and enzyme-linked antibodies. After a45-minute incubation at room temperature, the wells are washed with waterto remove unbound-labeled antibodies. A solution of TMB Reagent isadded and incubated for 20 minutes, resulting in the development of a bluecolor. The color development is stopped with the addition of Stop Solution,and the color is changed to yellow and measured spectrophotometricallyat 450 nm
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