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Human Probable arylformamidase (AFMID) ELISA Kit

Human Probable arylformamidase (AFMID) ELISA Kit

Cat no: KTE60956

Supplier: Abbkine Scientific Co.Ltd.
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Arylformamidase (AFMID) is the second enzyme of the kynurenine pathway metabolizing tryptophan to nicotinic acid and nicotinamide adenine dinucleotide cofactors. Inhibition of AFMID by organophosphorus insecticides in developing chicken embryos is correlated with lowered NAD levels and severe teratogenesis. The cDNA sequence previously identified for mouse liver AFMID was cloned and expressed in Escherichia coli. Residues identified as potential catalytic triad members through sequence motif and homology modeling were mutated to alanine to probe their contributions to enzyme activity. The wild-type and mutant AFMIDs were expressed as amino terminal 6 x His-tagged recombinant proteins to facilitate purification. Three chromatography steps isolated highly purified proteins for enzyme activity comparisons.
Catalogue number: KTE60956
Reactivities: Human
Applications: ELISA
Size: 48T, 96T, 96T*5, 96T*50
Accession: Q63HM1
Gene: 125061
Additional info: Human Probable arylformamidase (AFMID) ELISA Kit has high sensitivity and excellent specificity for detection of Human AFMID. No significant cross-reactivity or interference between Human AFMID and analogues was observed.

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