The Testosterone EIA is based on the principle of competitive binding between Testosterone in the test specimen and Testosterone-HRP conjugate for a constant amount of rabbit anti-Testosterone. In the incubation, goat anti-rabbit IgG-coated wells are incubated with 10 μl of Testosterone standards, controls, patient samples, 100 μl Testosterone-HRP conjugate reagent and 50 μl rabbit anti-Testosterone reagent at 37