Hyaluronic Acid Binding Protein (Hyaluronan Binding Protein, HABP)

Hyaluronan binding protein 1 (HABP1), also known as C1qBP/C1qR and p32, is a ubiquitous acidic glycoprotein that functions in spermatogenesis and as a receptor for proinflammatory molecules (1, 2). HABP1 is synthesized with a 71 amino acid (aa) N-terminal preproprotein and a 208 aa mature region (3). The 3kD mature mouse HABP1, which contains a MAM33-like sequence, shares 90% and 99% aa sequence identity with human and rat HABP1, respectively. HABP1 assembles into a doughnut shaped trimer, with negatively charged residues asymmetrically distributed on one face lining the channel of the complex (4). HABP1 can be cleaved by cell surface MMP-14/MT1-MMP, a protease important in angiogenesis and tumor metastasis (5). Cell surface HABP1 binds a wide range of extracellular molecules, including hyaluronan, vitronectin, complement component C1q, HMW kininogen, and bacterial and viral proteins (2, 6-9). Within the cell, HABP1 binds to molecules containing the C1q globular domain, multiple isoforms of PKC, mitochondrial Hrk, the cytoplasmic tails of adrenergic and GABA-A receptors, the mRNA splicing factor ASF/SF2, and the CBF transcription factor (10-16). Apoptosis and direct phosphorylation by Erk1/2 induces HABP1 translocation to the nucleus (17).\n\nApplications: \nSuitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. \n\nAlso Available:\nH7980-30: Hyaluronic Acid Binding Protein (HABP)\nH7980-30A: Hyaluronic Acid Binding Protein (HABP) Pab Rb xBo\nH7980-35: Hyaluronic Acid Binding Protein (HABP) (Biotin)

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SPECIFICATIONS

Catalog Number

H7980-30A

Size

1ml

Applications

ELISA, ICC, IHC, WB

Hosts

Rabbit

Reactivities

Mouse, Rat, Bov

Form

Supplied as a liquid, serum, before the addition of glycerol to 40%.

P Type

Pab

Purity

Neat serum

Isotype

IgG

References

1. Thakur, S.C. et al., 2006, J. Androl. 27:604. 2. Peerschke, E.I.B. and B. Ghebrehiwet, 2007, Immunobiology 212:333. 12. Sunayama, J. et al., 1994, Cell Death Differ. 11:771. 3. Lynch, N.J. et al., 1997, FEBS Lett. 418:111. 13. Pupo, A.S. and K.P. Minneman, 2003, J. Recept. Signal Transduct. 4. Jiang, J. et al., 1999, Proc. Natl. Acad. Sci. 96:3572. Res. 23:185. 5. Rozanov, D.V. et al., 2002, J. Biol. Chem. 277:9318. 14. Schaerer, M.T. et al., 2001, J. Biol. Chem. 276:26597. 6. Deb, T.B. and K. Datta, 1996, J. Biol. Chem. 271:2206. 15. Peterson-Mahrt, S.K. et al., 1999, EMBO J. 18:1014. 7. Lim, B.L. et al., 1996, J. Biol. Chem. 271:26739. 16. Chattopadhyay, C. et al., 2004, Nucleic Acids Res. 32:3632. 8. Grebrehiwet, B. et al., 1994, J. Exp. Med. 179:1809. 17. Majumdar, M. et al., 2002, Biochem. Biophys. Res. Commun. 9. Waggoner, S.N. et al., 2007, J. Leukoc. Biol. 82 epub. 291:829. 10. Innamorati, G. et al., 2006, Cell. Signal. 18:761. 11. Robles-Flores, M. et al., 2002, J. Biol. Chem. 277:5247. 12. Laemmli, U.K., Nature (Lond.) 227: 680-685 (1970). 13. Rao, C.M., Salotra, P., Datta, K.: Possible Role of the 34-Kilodalton Hyaluronic Acid-Binding Protein in Visceral Leishmaniasis. Journal of Parasitology 85: 682-687 (1999).

Additional Info

Recognizes Hyaluronic Acid Binding Protein (HABP). Species Crossreactivity: Mouse and rat.