Hyaluronidase, Bovine, Highly Purified

Hyaluronidase catalyzes the random hydrolysis of 1,4-linkages between 2-acetamido-2-deoxy-beta-D-glucose and D-glucose residues in hyaluronate. Hyaluronate 4-glycanohydrolase (EC 3.2.1.35) catalyzes the depolymerization of mucopolysaccharides, hyaluronic acid, and the chondroitin sulfates A and C. The enzyme is widely distributed in animal tissues but is found in great concentrations in the bovine and ovine testes. It is also produced by a number of bacteria. Hyaluronidase from bovine testes has a molecular weight of 55,000. Purified hyaluronidase is used clinically for the intradermal administration of large volumes of fluid when intravenous injections are contraindicated. The enzyme is administered prior to or simultaneously with the fluid and it facilitates absorption of the fluid.\n\nActivity (USP/NF units/mg dw):\n(same/more than)3000u/mg\n\nAbsorbance (A280@mg/ml):\nAs Reported\n\nUnit Definition:\nOne unit is based on the change in absorbency (turbidity) at 540nm of an internal standard.\n\nQuality Control:\nSDS-PAGE\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Prepare fresh before use..\n\nCertificate of Origin:\nThe raw animal product (bovine glands, organs and tissue) used in the manufacturing of Hyaluronidase is derived from materials of USDA-approved origin. It was collected in USDA or equivalent approved facilities and inspected to be free of disease.

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SPECIFICATIONS

Catalog Number

H7981

Size

5KU

Form

Supplied as a dialyzed, lyophilized powder

Purity

Chromatographically purified

References

1. Human Biochemistry; Orten, J.M., and Neuhaus, O.W.; 10th edition, Pg. 411, 1982. Mosby, St. Louis. 2. Methods of Enzymatic Analysis, Bergmeyer, H.U., ed., Vol. 2, pp. 944-948, Academic Press, New York, 1974. 3. Borders, C., and Raftery, M.. J Biol Chem 243, 3756, 1968. 4. Khorlin, A., Vikha, I., and Milishnikov, A.. F.E.B.S. Lett. 31, 107, 1973. 5. DeSalequi, M., Plonska, H., and Pigman, W. Arch Biochem Biophys 121, 548, 1967. 6. Warren, G., Seifter, J., and Glassman, J. Nature 194, 770, 1962. 7. Ludowieg, J., Vennesland, B., and Dorfman, A. J Biol Chem 236, 333, 1961. 8. Rappaport, M., Meyer, K., and Linker, A. J Am Chem Soc 73, 2416, 1951. 9. Yang, C., and Srivastav, P. J Biol Chem 250, 79, 1975.

Alternative Names

Bovine testes, Hyaluronate 4-glycanohydrolase, EC=3.2.1.36