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Hybridization Solution (For use in Northern Blot)

Cat no: H7990

Hybridization Solution (For use in Northern Blot)

Description:\nHybridization Solution is a ready to use solution for nuclear acid hybridization. The solution is a proprietary formulation. Each lot of Hybridization Solution is tested by Northern and Southern hybridization to ensure quality.\n\nProcedure:\n1. Heat the solution at 65 degrees C to dissolve the precipitate completely if there are precipitate formed at room temperature.\n2. Prehybidize the membrane (Northern blot or Southern blot or RNA arrays) with solution at 65 degrees C for 2-4 hours at the about amount of 0.2ml per cm2 membrane\n3. After prehybridization, change the solution with fresh Hybridization Solution at 0.1ml per cm2 membrane, and keep at 65 degrees C.\n4. Heat your probe (either radioactive or non-radioactive probes is ok) at 95 degrees C for 3-5 minutes, and transfer on ice immediately for 5 minutes.\n5. Briefly spin down the probe, and add into the Hybridization Solution with the membrane. \n6. Hybridize the membrane with the probe for from 2 hours to over night. \n7. After hybridization, remove the solution,\n\nFor Radioactive Probe:\n

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SPECIFICATIONS

Catalog Number

H7990

Size

250ml

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