Hydrogen Peroxide Cell-Based Assay Kit

It is well established that H2O2 is a cytotoxic agent but evidence also suggests that H2O2 may be an important regulator of eukaryotic signal transduction. Cayman's H2O2 Cell-Based Assay provides a simple fluorometric method for the sensitive quantitation of H2O2 in cultured cells. H2O2 is detected using 10-acetyl-3,7-dihydroxyphenoxazine (ADHP), a highly sensitive and stable probe for H2O2. In the presence of horseradish peroxidase, ADHP reacts with H2O2 with a 1:1 stoichiometry to produce highly fluorescent resorufin (excitation = 530-560 nm; emission = 590 nm). Catalase, an H2O2 scavenger, is included in the kit for checking specificity of the assay.

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SPECIFICATIONS

Catalog Number

600050

Form

96 Well

P Type

Assay Kits

Weight

0

Storage Temp

-20

Shipping Temp

-20

Additional Info

Hydrogen peroxide (H2O2) is a natural by-product of oxygen metabolism. It has strong oxidizing capacity and is thus considered a highly reactive oxygen species. It is well established that H2O2 is a cytotoxic agent but evidence also suggests that H2O2 may be an important regulator of eukaryotic signal transduction. Cayman's H2O2 Cell-Based Assay Kit provides a simple fluorometric method for the sensitive quantitation of H2O2 in cultured cells. H2O2 is detected using 10-acetyl-3,7-dihydroxyphenoxazine (ADHP), a highly sensitive and stable probe for H2O2. In the presence of horseradish peroxidase, ADHP reacts with H2O2 with a 1:1 stoichiometry to produce highly fluorescent resorufin (excitation = 530-560 nm; emission = 590 nm). Catalase, an H2O2 scavenger, is included in the kit for checking specificity of the assay.