Hypoxia Inducible Factor 3a (HIF-3a)

One of the most important factors in the cellular response to hypoxia is hypoxiainducible\nfactor (HIF), which transcriptionally activates genes encoding proteins that mediate adaptive responses to reduced oxygen availability. HIF is a heterodimer consisting of one of three subunits (HIF1-a, HIF2-a, or HIF3-a) bound to the aryl hydrocarbon receptor nuclear translocator (ARNT), which is also known as HIF1-b. HIF-a is a member of the basic helix-loop-helix (bHLH) superfamily, in which the HLH domain mediates subunit dimerization\nwhile the basic domain binds to DNA. HIF target genes play critical roles in metabolism, angiogenesis, cell proliferation, and cell survival; in fact, HIF3-a may be a marker for tumor growth and angiogenesis . Examples of HIF target genes include VEGF, glucose transporter 1 (GLUT1), and EPO. HIF binds to the hypoxia-responsive element, which contains the core recognition sequence 5'-TACGTG-3', in the cis-regulatory regions of hypoxiainducible genes. Transcriptional activation by HIF is linked to its ability to recruit coactivator proteins such as\nCREB-binding protein (CBP), p300, steroid receptor coactivator-1, and translation initiation factor 2. Hif3a may be a marker for tumor growth and angiogenesis.\n\nApplications: \nSuitable for use in ELISA. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:7000-1:30,000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

H9810-05

Size

100ug

Applications

ELISA

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. No stabilizing proteins added.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. William K. Chan, Guang Yao, Yi-Zhong Gu, and Christopher A. Bradfield, (1999) Cross-talk between the Aryl Hydrocarbon Receptor and Hypoxia Inducible Factor Signaling Pathways. J Biol Chem. 274;17, 12115-12123. 2. Erbel PJ, Card PB, Karakuzu O, Bruick RK, Gardner KH. (2003) Structural basis for PAS domain heterodimerization in the basic helix--loop--helix-PAS transcription factor hypoxia-inducible factor. Proc Natl Acad Sci U S A. 100(26):15504-9. 3. Makino Y, Kanopka A, Wilson WJ, Tanaka H, Poellinger L. (2002) Inhibitory PAS domain protein (IPAS) is a hypoxia-inducible splicing variant of the hypoxia- inducible factor-3alpha locus. J Biol Chem. 277(36):32405-8. 4. Luo JC, Shibuya M. (2001) A variant of nuclear localization signal of bipartite-type is required for the nuclear translocation of hypoxia inducible factors (1alpha, 2alpha and 3alpha). Oncogene. 20(12):1435-44.

Additional Info

Recognizes mouse HIF-3a. Species sequence homology: Rat