IDH1, Recombinant, Human, FLAG-Tag (Isocitrate Dehydrogenase [NADP] Cytoplasmic, IDH, Cytosolic NADP-isocitrate Dehydrogenase, IDP, NADP(+)-specific ICDH, Oxalosuccinate Decarboxylase, PICD)

IDH1 belongs to two distinct subclasses. The protein is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. This protein contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.\n\nSource:\nRecombinant corresponding to aa1-414 from human IDH1, fused to FLAG-tag at C-terminal, expressed in Sf9 insect cells via a baculovirus expression system.\n\nMolecular Weight:\n~47.6kD\n\nApplications: \nSuitable for use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

137303

Size

10ug

Form

Supplied as a liquid in 45mM Tris-HCl, pH 8.0, 124mM sodium chloride, 2.4mM potassium chloride, 90ug/ml FLAG peptide, 3mM DTT, 10% glycerol.

Purity

Purified (~80%)

References

1. Dang, L., et al. (2010). Trends Mol. Med. 16(9):387-97. 2. Paschka, P., et al. (2010). J. Clin. Oncol. 28(22):3636-43 3. Fu, Y., et al. (2010). Biochem. Biophys. Res. Commun. 397(2):127-30.