IgG1 Murine Negative Control (FITC)

Applications:\nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilution: \nFlow Cytometry: 10ul labels 10e6 cells or 100ul whole blood.\nOptimal dilutions to be determined by the researcher.\n\nSource: Tissue culture supernatant\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

I1904-78R3

Size

100Tests

Applications

FC

Reactivities

Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.1% sodium azide. Labeled with FITC.

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1

References

1. Kupatt, C. et al. (2000) c7E3Fab reduces postischemic leukocyte-thrombocyte interaction mediated by fibrinogen. Implications for myocardial reperfusion injury Arterioscler. Thromb. Vasc. Biol.20: 2226-32. 2. Dalli, J. et al. (2008) Annexin 1 mediates the rapid anti-inflammatory effects of neutrophil-derived microparticles. Blood. 112: 2512-2519.

Additional Info

Negative on all human cells and cell lines tested. Also suitable for use as a negative control with bovine, ovine, porcine, equine, canine, lapine and guinea pig tissues. Recognizes a rat cell surface marker and therefore cannot be used as a negative control in rat.