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IgG1 Negative Control (Azide Free)

Cat no: I1904-76J

IgG1 Negative Control (Azide Free)

Applications:\nSuitable for use in Flow Cytometry, Immunohistochemistry, ELISA. Other applications have not been tested. \n\nRecommended Dilutions:\nFlow Cytometry: Neat, Use 10ul of the recommended dilution to label 10e6 cells in 100ul.\nImmunohistochemistry: Frozen.\nOptimal dilutions to be determined by researcher.\n\nRecommended Secondary Reagents:\nGoat Anti-rat IgG F(ab')2 labeled with FITC (mouse absorbed)\nGoat anti-rat IgG F(ab')2 labeled with RPE (mouse adsorbed)\n\nFusion Partners: Spleen cells from immunized PVG-RT1u rat were fused with cells of the rat Y3/Ag1.2.3 myeloma cell line.\n\nSource: Tissue culture supernatant\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I1904-76J

Size

1mg

Applications

ELISA, FC, IHC

Hosts

Rat

Reactivities

Mouse

Form

Supplied as a liquid in PBS.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

Additional Info

Recognizes mouse IgG1. For use on mouse tissues or cells as a rat IgG1 isotype negative control for the measurement of non-specific binding of rat monoclonal antibodies of isotype IgG1. Species Crossreactivity: Recognizes human CD4, a cell surface marker. Cannot be used as a negative control with human.

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